Keywords
Loop-mediated isothermal amplification
qPCR
How to Cite
Abstract
Dengue viruses are mainly transmitted by the Aedes aegypti mosquito which grows in breeding containers maintained by rain or human activity. The Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) reaction was found more sensitive and can detect 10-copies of RNA template whereas; real-time PCR (qPCR) had detection limits of 100 copies. To detect the dengue serotypes from wild-caught Aedes aegypti mosquitoes using a molecular technique RT-LAMP, and compare with qPCR to detect the same dengue serotype. This prospective analytical study was conducted at the Department of Virology, BSMMU, Dhaka, Bangladesh from January 2017 to December 2017. RT-LAMP was performed to detect dengue serotypes from wild-caught mosquitoes. Four different visual detection methods of RT-LAMP were conducted simultaneously. To confirm the test result, qPCR was done. Out of the 1348 wild-caught mosquitoes, 217(16.10%) were found positive for the dengue virus by RT-LAMP assay. Among this DEN-2 serotype was by far the highest percentage 184(84.79%) whereas, DEN-1 was 33(15.21%). The RT-LAMP is a relatively inexpensive, rapid, and simple tool for the accurate detection and serotyping of DENV from wild-caught mosquitoes.
References
Armstrong, P. M., & Rico-Hesse, R. (2003). The efficiency of dengue serotype 2virus strains to infect and disseminate in Aedes aegypti. American Journal of Tropical Medicine & Hygiene,68, 539–44.
Bonilauri, P., Bellini, R., Calzolari, M., Angelini, R., Venturi, L., Fallacara, F., Cordioli, P., Angelini, P., Venturelli, C., & Merialdi, G. (2008). Chikungunya virus in Aedes albopictus, Italy. Emerging Infectious Diseases, 14, 852.
Coker, R. J., Hunter, B. M., Rudge, J. W., Liverani, M., & Hanvoravongchai, P. (2011). Emerging infectious diseases in southeast Asia: regional challenges to control. The Lancet, 377, 599–609.
Dash, P. K., Parida, M. M., Saxena, P., Abhyankar, A., Singh, C. P., Tewari, K. N., et al. (2006). Re-emergence of dengue virus type 3 (subtype III) in India: Implications for increased incidences of DHF and DSS, VirologyJournal, 3(13), 1–10.
Fan, W., Yu, S., & Cosgriff, T. M. (1989). The reemergence of dengue in China. Reviews of Infectious Diseases, 11, S847–S853.
Hayashi, N., Arai, R., Tada, S., Taguchi, H., & Ogawa, Y. (2007). Detection and identification of Brettanomyces/Dekkerasp. Yeasts with a loop-mediated isothermal amplification method. Food Microbiology, 24, 778–785.Dengue Serotype Detection from Wild Caught Mosquito
Ilkal, M. A., Dhanda, V., Hassan, M. M., Mavale, M., Mahadev, P. V. M., Shetty, P. S. et al. (1991). Entomological investigations during outbreaks of dengue fever in certain villages in Maharastra state. Indian Journal of Medical Research, 93, 174–8.
Karabatsos, N. (1975). Antigenic relationships of group A arboviruses by plaque reduction neutralization testing. American Journal of Tropical Medicine & Hygeine, 24, 527–532.
Lakshmi, V., Neeraja, M., Subbalaxmi, M. V., Parida, M. M., Dash, P. K., Santhosh, S. R., & Rao, P. V. (2008). Clinical features and molecular diagnosis of Chikungunya fever from South India. Clinical infectious diseases: An official publication of the Infectious Diseases Society of America, 46(9), 1436–1442.
Lau, Y. L., Lai, M. Y., Teoh, B. T., Abd-Jamil, J., Johari, J., Sam, S. S., Tan, K. K., & AbuBakar, S. (2015). Colorimetric detection of dengue by single tube Reverse-Transcription-Loop-Mediated isothermal amplification. PloS one, 10(9), e0138694.
Lu, L., Lin, H., Tian, L., Yang, W., Sun, J., & Liu, Q. (2009). Time series analysis of dengue fever and weather in Guangzhou, China. BMC Public Health, 9, 395.
Mutsuddy, P., Tahmina, J, S., Shamsuzzaman, A. K. M., Kaisar, S. M. G., Khan, M. N. A., and Dhiman, S. (2019). Dengue situation in Bangladesh: An epidemiological shift in terms of morbidity and mortality. Canadian Journal of Infectious Diseases and Medical Microbiology, 2019, 1-13 https://doi.org/10.1155/2019/3516284.
Pan American Health Organization. (1994). Dengue and Dengue Haemorrhagic Fever: Guidelines for prevention and control. Washington D.C. Scientific publication No. 548.18.
Parola, P., de Lamballerie, X., Jourdan, J., Rovery, C., Vaillant, V., Minodier, P., Brouqui, P., Flahault, A., Raoult, D., & Charrel, R. N. (2006). Novel Chikungunya virus variant in travelers returning from Indian Ocean islands. Emerging Infectious Diseases, 12(13), 1493–1498.
Peters, C. J. (1997). Viral Hemorrhagic Fevers. In Viral Pathogenesis. Nathanson N. (Editor). Philadelphia: Lippincott-Raven Publishers, 779-799.
Powers, A. M., Brault, A. C., Tesh, R. B., & Weaver, S. C. (2000). Re-emergence of Chikungunya and O’nyong-nyong viruses: Evidence for distinct geographical lineages and distant evolutionary relationships. Journal of General Virology, 81, 471–479.
Sheng-feng, H., Miao, L., Lan-lan, Z., Shi-miao, L., Ze-xia, L., Jie-ying, P., Jin-sheng., & Wen, X. H. (2009). Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1–4. BMC Microbiology, 15(1), 77. Available at: http://www.biomedcentral.com/1471-2180/15/265.
Sithiprasasna, R., Strickman, D., Innis, B. L., & Linthicum, K. J. (1994). ELISA for detecting dengue and Japanese encephalitis viral antigen in mosquitoes. Annals of Tropical Medicne & Parasitology, 88, 397–404.T. Rawnuck et al
Telles. J. N., Le R. K., Grivard, P., Vernet, G., & Michault, A. (2009). Evaluation of real-time nucleic acid sequence-based amplification for detection of chikungunya virus in clinical samples. Journal of Medical Microbiology, 58, 1168–1172.
Tsetsarkin, K. A., Chen, R., Leal, G., Forrester, N., Higgs, S., Huang, J., & Weaver, SC, 2011. Chikungunya virus emergence is constrained in Asia by lineage-specific adaptive landscapes. Proceedings of the National Academy of Sciences, 108, 7872–7877.
Vazeille, M., Rosen, L., Mousson, L., & Failloux, A. B. (2003). Low oral receptivity for dengue type 2 viruses of Aedes albopictus from South-east Asia compared with that of Aedes aegypti. American Journal of Tropical Medicine & Hygiene, 68, 203-208.
Victor, T. J., Malathi, M., Gurusamy, D., Desai, A., Ravi. V., Narayanasamy, G., et al. (2002). Dengue fever outbreaks in two villages of Dharmapuri district in Tamil Nadu. Indian Journal of Medical Research, 116, 133–139.